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Chi-squared goodness-of-fit test was performed to assess for difference in frequencies between the groups in (f) and (g).Ĭoncentrations of 45 immune mediators were quantified using a 45-plex microbead-based immunoassay. ( f) and ( g) Cumulative analysis of patient frequency data of CECs staining positive for endothelial activation markers, ICAM1, SELP, and/or CX3CL1. Kruskal-Wallis test was performed ( b-e) to test for difference between the groups with Dunn’s multiple comparison test carried out for pairwise testing post hoc. ( d) and ( e) Boxplots extending from 25th to 75th percentile with bar showing mean and whiskers indicating the minimum and maximum number of CECs per million PBMCs from each group staining positive for endothelial activation markers ICAM1, SELP, or CX3CL1. ( b) and ( c) Scatterplot visualization of the number of CECs and endothelial progenitor cells (EPCs) per million PBMCs identified from each sample with mean and standard error of mean for each group shown. ( a) Using flow cytometry, CEC populations were gated from peripheral blood mononuclear cells (PBMCs) using a strategy involving positive (nuclear stain and CD31) and negative (CD45 and CD133) markers before characterization with three separate markers of endothelial activation. Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore.Yong Loo Lin School of Medicine, National University of Singapore, Singapore.Department of Medicine, National University Hospital, Singapore.Department of Infectious Diseases, Changi General Hospital, Singapore.National Heart Centre Singapore, Singapore.
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Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, ANA Futura, Campus Flemingsberg, Sweden.Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore.National Centre for Infectious Diseases, Singapore.Department of Biological Sciences, National University of Singapore, Singapore.Singapore Immunology Network, Agency for Science, Technology and Research, Singapore.A*STAR ID Labs, Agency for Science, Technology and Research, Singapore.Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.